Fig 3. Temperature shift experiments point to the wanderer stage of development as the most sensitive stage regulating ventral HW eyespot size and brightness.

A, B) Animals were reared at either 17°C (brown) or 27°C (green) for most of their development with the exception of a single 48hr window where they were reared at the other temperature. C, D) Estimated eyespot size or eyespot center size (E, F) for wings with constant area of 250 mm². G, H) Eyespot center K value (measure of darkness) of animals shifted at different stages during their development. DS: non-shifted Dry Season animals; WS: non-shifted Wet Season animals; L5-2: animals shifted at start of stage 2 during the 5^th instar (see Table 2 for stage description); L5-3: at start of stage 3 during the 5^th instar; W: at start of wanderer stage; PP: at start of pre-pupal stage; P1: at start of pupal stage 1; P2: at start of pupal stage 2. C, E, G) Most of development happened at 17°C. D, F, H) Most of development happened at 27°C. I) Representative DS control individual (left) versus DS shifted to higher temperatures at wanderer stage. J) Representative WS control individual (left) versus WS shifted to lower temperatures at wanderer stage. Error bars = 95% CI of the means. Experimental groups labeled with the same letter: “a”, “b”, “c” or “d” are not significantly different from each other, whereas groups labeled with a different letter are.