Fig 4. 20E hormone titers are higher in WS forms at the wanderer stage of development and the 20E receptor (EcR) is present in hindwing but absent from forewing eyespot centers at that stage.
A) Titers of 20-hydroecdysone (20E) at several stages of larval, pre-pupal and pupal development. DS forms = brown line; WS forms = green line; Stages described in Table 2. Error bars correspond to 95% CI for means. B) During the wanderer (W) stage of development EcR (green) is expressed in hindwing eyespot centers of both seasonal forms (WS, DS) but not in forewing eyespots, whereas a second eyespot-associated protein, spalt (sal; red), is expressed in all eyespot centers. C) Earlier during the 5th instar, however, EcR is expressed in both forewing (left) and hindwing (right) eyespot centers on the ventral surface. Arrows point to the Cu1 eyespot.