Fig 5. Manipulations of ecdysone signaling during the wanderer stages of development alters hindwing eyespots more extensively than forewing eyespots.

Injections of 20-hydroxyecdysone (20E) and vehicle (V) were performed in DS forms (brown). Injections of Cucurbitacin B (CucB) and vehicle were performed in WS forms (green). Measurements of eyespot size (A, B), eyespot center size (C, D) and eyespot center darkness (K value) were performed in forewing eyespots (A, C, E; left graphs) and hindwing eyespots (B, D, F; right graphs). Error bars = 95% CI of means. Significant differences between treatments are represented by asterisks: *, p < 0.05; **, p < 0.01, ***, p < 0.001, ns = non significant. Values for trait size in A-D are evaluated at a wing area of 227 mm².