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. 2015 Sep 25;10(9):e0138515. doi: 10.1371/journal.pone.0138515

Fig 2. SALL4 induces the EMT and invasive phenotype in endometrial cancer cells.

Fig 2

(A) N-cadherin, E-cadherin, SALL4A and SALL4B were assessed by western blotting in endometrial cancer cell lines: AN3CA, KLE, HEC1-B, HEC1-A, RL-952 and Ishikawa. (B) Cell morphology was examined in Ishikawa cells transfected with SALL4A or SALL4B expression vector and AN3CA cells transfected with SALL4-sh1 or SALL4-sh2. (C)(D) The protein and mRNA levels of E-cadherin and N-cadherin were analyzed in Ishikawa cells transfected with SALL4A or SALL4B expression vector and AN3CA cells transfected with SALL4-sh1 or SALL4-sh2. (E) Cell migration and invasion of Ishikawa or AN3CA were examined through wound healing assay and transwell invasion assay. (F) The degree of migration and invasion in Ishikawa and AN3CA cells was analyzed. *P<0.01.