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. 2015 Sep 25;10(9):e0138515. doi: 10.1371/journal.pone.0138515

Fig 4. ABCB1 is critical for SALL4-induced drug resistance.

Fig 4

(A) Western blotting showed that ectopic overexpression of SALL4 upregulated ABCB1 protein expression in Ishikawa cells and knockdown of SALL4 in AN3CA cells downregulated ABCB1 expression. (B)(C) Ishikawa cells transfected with SALL4A or SALL4B vector or control vector were transfected with si-ABCB1 or control siRNA. Drug sensitivity of Ishikawa cells to carboplatin was examined by cell viability assay and clone formation assay. (D)(E) The protein and mRNA levels of ABCB1 were assessed by western blotting and qRT-PCR. **P<0.01.