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. 2015 Sep 25;10(9):e0138688. doi: 10.1371/journal.pone.0138688

Fig 3. B cells following hepatic IRI.

Fig 3

Following 40 minutes left lobe ischemia, WT mice were allowed to reperfuse for 3, 6 or 12 hours. Mice were then sacrificed and their ischemic left lobes digested. The isolated immune cells were analysed by flow cytometry. There was a significant influx of immune cells (defined as CD45+ cells) into the injured left lobe [Kruskall- Wallis p = 0.0080] (A). B cells were defined as CD3-CD19+ gated lymphocytes. The number of live B cells was found to decrease significantly with time following reperfusion [Kruskall- Wallis p = 0.0043] (B); this was predominantly due to cell death (as defined by positive staining for a live-dead marker) [Kruskall- Wallis p = 0.0060] (C).