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. 2015 Sep 25;10(9):e0139121. doi: 10.1371/journal.pone.0139121

Fig 3. Effect of nanocurcumin on p-300 HAT and HDAC activities in HVCM cells under hypoxia:

Fig 3

Histone acetylation by p-300 was up-regulated with a corresponding decline in HDAC activity in cardiomyocytes under hypoxia depicting that hypoxia induced hypertrophy dependent upon p-300 HAT and HDAC activity (A and C). Nanocurcumin treatment significantly down-regulated p-300 HAT and up-regulated HDAC activity in HVCM cells under hypoxia compared to cells exposed to hypoxia only. This was further confirmed by western blot analysis of GATA-4 and p-GATA-4 levels along with acetylated histone 3 and 4 (B). However, curcumin treatment did not prevent histone acetylation under hypoxia. Also, nanocurcumin or curcumin treated cells did not show change in p-300 HAT activity under normoxia. Values are mean ± SD, significant values represented as @@ p≤0.01 vs normoxia, *p≤0.05 vs hypoxia and #p≤0.01 vs hypoxia. Non-significant changes are depicted as ns. Scale bar represents 50μm.