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. 2015 Sep 25;10(9):e0139121. doi: 10.1371/journal.pone.0139121

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© 2015 Nehra et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) MnSOD activity showed gradual decline with increment of duration of hypoxia as shown by fluorescent staining. (B) Curcumin treatment did not show significant restoration in MnSOD activity when compared to hypoxia control cells. (C) Treatment with nanocurcumin restored MnSOD activity in time dependent manner. Nanocurcumin significantly restored MnSOD activity in HVCM cells under hypoxia than curcumin treated cells under hypoxia when compared to cells exposed to hypoxia only. Random fields were captured by fluorescent microscope at 40 X magnification. At least 70 images were taken for each group and fluorescence intensity was quantized by using ImageJ software. Bar represents 10 μm.