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. 2015 Sep 25;11(9):e1005185. doi: 10.1371/journal.ppat.1005185

Fig 3. Localization of early secretory membranes in EMCV-infected cells.

Fig 3

HeLa R19 cells were mock-infected or infected with EMCV-2C-HA (A-C) or EMCV (D-F) at MOI 10. 6 h later, cells were fixed and co-stained with antibodies against viral 3AB or HA as RO markers and antibodies against endogenous GM130 (cis-Golgi marker; A), TGN46 (trans-Golgi marker, B), ERGIC53 (ERGIC marker, C), Sec13 (ER exit site marker, D) or calreticulin (ER marker, E and F). Nuclei were stained with DAPI (blue). The crop panels at the bottom depict enlargements of boxed areas. Scale bars represent 10 μm.