Fig 4. Sarcoplasmic reticulum Ca²⁺ loading properties.

Sarcoplasmic reticulum Ca²⁺ loading data and relative protein content with associated standard curves for both male (A-B) and female (C-D) offspring. The relative area of individual caffeine induced force responses, indicative of the amount of Ca²⁺ loaded into the SR, showed no significant differences between treatments for the load times investigated (male: C, n = 10; NR, n = 10, and female: C, n = 10; NR, n = 10). Quantity of SERCA2a and CSQ2 when normalised to total protein in Stain Free gel (see methods; myosin heavy chain (MHC) indicative of amount total protein loaded) showed no significant difference between treatments for male (C n = 10, NR n = 8) or female offspring (C n = 8, NR n = 10). Values above standard curves indicate amount (μg) wet muscle loaded.