Table 2. Minimum inhibitory concentrations (MICs) of ITZ and TBZ in the airborne thermotolerant fungal isolates determined by the CLSI M38-A2 broth dilution method.
| Type | Species | Strain ID | GenBank accession number (ITS/benA/cyp51A) | Location | Day (mm/dd/yyyy) | Type of agar plate (concentration, μg/mL) a | ITZ MIC (μg/mL) b | TBZ MIC (μg/mL) b |
|---|---|---|---|---|---|---|---|---|
| Sampled strains | ||||||||
| Aspergillus niger | 13L06I1 | KP724986/KP24980 | University | 03/14/2014 | ITZ (16) | 2.0 | 4.0 | |
| Paecilomyces variotii | 17L16T1 | KP724987 | University | 06/13/2014 | TBZ (16) | 0.75 | ≥32 | |
| Paecilomyces variotii | 17L16T2 | KP724988 | University | 06/13/2014 | TBZ (16) | 0.75 | ≥32 | |
| Paecilomyces variotii | 17L16T3 | KP724989 | University | 06/13/2014 | TBZ (16) | 0.75 | ≥32 | |
| Paecilomyces variotii | 17L16T4 | KP724990 | University | 06/13/2014 | TBZ (16) | 1.0 | ≥32 | |
| Paecilomyces variotii | 17L16T5 | KP724991 | University | 06/13/2014 | TBZ (16) | 1.0 | ≥32 | |
| Aspergillus fumigatus | 24L15T1 | KP724998/KP24983/KP725004 | University | 09/12/2014 | TBZ (4) | 1.5 | 2.0 | |
| Paecilomyces variotii | 25L15T1 | KP724999 | University | 10/06/2014 | TBZ (4) | 0.75 | ≥32 | |
| Aspergillus fumigatus | 21L05I1 | KP724992/KP24981/KP725002 | Apple farm | 08/05/2014 | ITZ (4) | 1.5 | 2.0 | |
| Aspergillus fumigatus | 21L05I2 | KP724993/KP24982/KP725003 | Apple farm | 08/05/2014 | ITZ (4) | 1.0 | 2.0 | |
| Coprinellus curtus | 21L05I3 | KP724994 | Apple farm | 08/05/2014 | ITZ (4) | n.d. | n.d. | |
| Coprinopsis cinerea | 21L06I1 | KP724995 | Apple farm | 08/05/2014 | ITZ (16) | n.d. | n.d. | |
| Coprinopsis cinerea | 21L06I2 | KP724996 | Apple farm | 08/05/2014 | ITZ (16) | n.d. | n.d. | |
| Unidentified | 21L15T1 | Apple farm | 08/05/2014 | TBZ (4) | n.d. | n.d. | ||
| Choanephora infundibulifera | 21L15T2 | KP724997 | Apple farm | 08/05/2014 | TBZ (4) | n.d. | n.d. | |
| Paecilomyces variotii | 26L15T1 | KP725000 | Apple farm | 10/08/2014 | TBZ (4) | 1.0 | ≥32 | |
| Unidentified | 05L15T1 | KP724985 | Rice farm | 12/13/2013 | TBZ (4) | n.d. | n.d. | |
| Unidentified | 22L15T1 | Rice farm | 08/05/2014 | TBZ (4) | n.d. | n.d. | ||
| Aspergillus tubingensis | 27L05I1 | KP725001/KP24984 | Rice farm | 10/08/2014 | ITZ (4) | 4.0 | 8.0 | |
| Quality control strains | ||||||||
| Candida parapsilosis | ATCC 22019 | - | - | - | - | 0.25 [0.12–0.5] c | 1.0 | |
| Candida krusei | ATCC 6258 | - | - | - | - | 0.5 [0.25–1.0] c | 4.0 | |
| Reference strains | ||||||||
| Aspergillus flavus | ATCC 204304 | - | - | - | - | 0.25 [0.25–0.5] c | 1.0 | |
| Aspergillus fumigatus | ATCC MYA 3626 | - | - | - | - | 1.0 [0.25–2.0] c | 2.0 | |
| Aspergillus fumigatus | ATCC MYA 4609 | - | - | - | - | 1.0 | 4.0 | |
| Aspergillus fumigatus | ATCC MYA 3627 | - | - | - | - | ≥32 [>16] c | 4.0 |
a Types of agar plates on which the strains were isolated.
b Median values of at least four replicated experiments were used.
c The values in brackets are the MIC ranges reported by the CLSI [40].
Abbreviations: ITZ, itraconazole; TBZ, tebuconazole; n.d., not determined due to difficulties in identification or difficulties in sporulation.