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. 2015 Sep 25;10(9):e0138892. doi: 10.1371/journal.pone.0138892

Fig 8. Major changes in the Ni-Sp185/333 protein repertoire may not occur until after the second challenge with V. diazotrophicus.

Fig 8

(A) Sea urchin 106 has a limited array of Ni-Sp185/333 proteins prior to immune challenge, with a few high MW proteins of basic pI (arrow). (B) After the first challenge with V. diazotrophicus, the Ni-Sp185/333 proteins appear as more acidic (arrows) plus proteins of ~60 kDa appear (arrow head). (C) After the second challenge with V. diazotrophicus, a significant change in the repertoire of Ni-Sp185/333 proteins shows a shift to more basic pI for both the large MW proteins (arrows), and the ~60 kDa proteins (arrowhead). These images were cropped on the bottom because they do not show spots of less than 50 kDa.