Table 2. Ni-Sp185/333 proteins isolated from immune activated sea urchins show a wide range of size and variable presence among animals.
| Animal | Immune challenge 1 | Ni-Sp185/333 protein sizes (kDa) 2 | Total protein yield % Sp185/333 3 |
|---|---|---|---|
| 1 | None | ~250, 200, 145, 85 | nd 4 |
| 5 | Vd | None | na |
| 6 | Vd | ~230, 200, 140, 82 | 25 μg/ml; 30% |
| 7 | None | ~250, 80 | 21 μg/ml; 45% |
| 8 | Vd | ~85, 50 | 9 μg/ml; 35% |
| 9 | Vd | ~200, 140, 85 | 6 μg/ml; 20% |
| 12 | Vd, Ec, L | None | na |
| 13 | No | ~250, 200, 85, 35 | nd |
| 15 | Vd | ~200, 140, 85 | 4 μg/ml; 28% |
| 17 | Vd, Ec, L | None | na |
| 30 | Vd | ~250, 185, 145, 80 | 25 μg/ml; 30% |
1 Vd, Vibrio diazotrophicus injection (104−106/ml wCF); Ec, E. coli injection (104 to 106/ml wCF); L, LPS injection (1–2 μg/ml wCF).
2Protein isolation by nickel affinity followed the protocol recommended by CloneTech.
3The percentage of Sp185/333 proteins of the total protein yield was estimated by densitometry of Western blots and corresponding protein gels; see Materials and Methods.
4nd, not done; na, not applicable.