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. 2015 Sep 25;10(9):e0139058. doi: 10.1371/journal.pone.0139058

Fig 1. Effect of the H29D mutation on the Ca2+ dependent activation of [3H]ryanodine binding to RyR2.

Fig 1

(A) [3H]ryanodine binding to cell lysate from HEK293 cells expressing the RyR2 WT or the H29D mutant was carried out at various Ca2+ concentrations (~0.2 nM to 0.1 mM), 100 mM KCl, and 5 nM [3H]ryanodine. Amounts of [3H]ryanodine binding at various Ca2+ concentrations were normalized to the maximal binding (100%). Data points shown are mean ± SEM from 3 separate experiments. (B,C) Immuno-blotting of RyR2 WT and the H29D mutant from the same amount of cell lysates using the anti-RyR antibody (34c) (n = 3).