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PA inhibits the polymerization of intracellular microtubules. (A) Immunoblot analyses of α-tubulin in the insoluble and soluble fractions of A549 and NCI-H1299 cells treated with PA (20 or 10 nM, respectively) for 24 or 48 h. Con, the mock-treated control. (B) Immunocytochemical staining for α-tubulin in A549 and NCI-H1299 cells treated with PA (20 or 10 nM, respectively) for 24 h. Nuclei are stained blue, while α-tubulin is shown in green.
