Figure 5.

Myricetin induces apoptosis on ovarian cancer cells in a p53-dependent pathway. (A) Myricetin decreased the expression of protein cmyc, and increased levels of protein p21 and p53. (B) Cells were treated with myricetin and p53 inhibitor pifithrin-α (PFT-α) for 24 h, stained with Hoechst 33342, apoptotic rate measured by fluorescence microscope. ^*p<0.05 as compared to myricetin-treated control. (C) Cells were treated with myricetin and PFT-α for 4 h, and caspase-3/7 enzymatic activity was determined using Caspase-Glo 3/7 assay kit. ^*p<0.05 as compared to myricetin-treated control. (D) Knockdown p53 resulted in abrogation of myricetin-increased levels of Bad, Bax, p21 and p53 and decreased levels of cmyc, Bcl-2 and Bcl-xl in OVCAR-3 cells. Cells were seeded in 60-mm dishes, incubated overnight, and then transfected with p53 siRNA or control siRNA. After 24 h, cells were treated with myricetin or DMSO. Cell lysates were collected for western blotting.