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. 2015 Sep 8;34(5):2790–2796. doi: 10.3892/or.2015.4250

Figure 2.

Figure 2

Effects of ferritin and doxorubicin on FTL expression in MCF-7. Cells were seeded in a 6-well culture plate for 24 h and starved for 24 h. The cells were treated with 25 nM human liver ferritin for 3–24 h (A). On day 1 of the combination treatment, the cells were pretreated with 25 nM human liver ferritin for 24 h, followed by 1 µM doxorubicin for 24 h on day 2 (B). The cytosol protein was analyzed for FTL by western blotting using β-actin as a loading control. The mRNA expression was analyzed for FTL gene by RT-PCR. The bars show mean ± SEM, each from three independent experiments. *P<0.05 vs. untreated control groups. FTL, ferritin light chain.