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. 2015 Aug 31;47(4):1249–1256. doi: 10.3892/ijo.2015.3145

Figure 3.

Figure 3

Sphere formation assay from UTSCC9 and UTSCC90. (A) Sphere formation of ALDH1high cells after cultivation in serum-free cell culture medium. (B) After ALDH1high, ALDH1low and parent 5×103 cells were cultured in 6-well ultra-low attachment dishes, total number of spheres per well was counted. The horizontal bars represent the mean expression levels; *P<0.05, **P<0.01, by unpaired t-test.