Figure 3.

Effects of SFRP2 on cell cycle in lung cancer cells. (A) Representative images of the cell cycle assays using flow cytometry in 95-D cells with or without SFRP2 knockdown at 48 h after transfection. (B) Representative images of cell cycle assays using flow cytometry in A549 cells with or without SFRP2 knock-in at 48 h after transfection. (C) Real-time RT-PCR detection of the cell cycle-related molecules in 95-D cells with or without SFRP2 knockdown. (D) Representative results of western blotting on Cyclin D1, Cyclin E1, CDK4, CDK6, p27 and β-actin in 95-D cells transfected by SFRP2 siRNA for 48 h. ov. and kn. represent overexpression and knockdown, respectively. Data are presented as mean ± SD. Statistical significance was evaluated using the Student's t-test. ^*P-value <0.05.