Table 4. Two-way hemagglutination inhibition assay of reference A(H7N9) viruses and LAIV reassortants.
| REFERENCE FERRET ANTISERUM | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| NL/219/03^ | ML/NL/12 | Anhui/1/13 | SH/2/13 | TW/1/13 | HK/5942/13 | CDC-LVRG1 | CDC-LV7A | Passage a | ||
| REFERENCE STRAINS | ||||||||||
| H7N7 | A/Netherlands/219/2003 | 160 b | 5 | 20 | 80 | 80 | 10 | 40 | 80 | EX/E2 |
| av-H7N3 | A/Mallard/Netherlands/12/2000 | 640 | 160 | 160 | 160 | 640 | 160 | 160 | 320 | E2/E2 |
| H7N9 | A/Anhui/1/2013 | 320 | 160 | 320 | 640 | 1280 | 160 | 640 | 640 | E2/E1 |
| H7N9 | A/Shanghai/2/2013 | 160 | 160 | 160 | 160 | 320 | 80 | 640 | 320 | E1/E1 |
| H7N9 | A/Taiwan/1/2013 | 320 | 320 | 320 | 640 | 1280 | 80 | 1280 | 640 | E1/E1 |
| H7N9 | A/Hong Kong/5942/2013 | 320 | 160 | 160 | 320 | 640 | 320 | 320 | 640 | M3/E1 |
| REASSORTANTS | ||||||||||
| H7N9 | A/Anhui/1/2013- CDC-LVRG1 | 320 | 160 | 160 | 640 | 640 | 160 | 640 | 320 | C1/E2 |
| H7N9 | A/Anhui/01/2013-CDC-LV7A | 320 | 320 | 320 | 640 | 1280 | 160 | 640 | 1280 | E2/E1// SPF7 |
| TEST ANTIGENS | ||||||||||
| H7N9 | A/Shanghai/1/2013 | 320 | 80 | 160 | 320 | 640 | 80 | 640 | 320 | E1/E1 |
| H7N9 | A/Jiangsu/1/13 | 320 | 160 | 320 | 640 | 640 | 160 | 1280 | 640 | E2/E1 |
| H7N9 | A/Zhejiang/2/2013 | 320 | 320 | 320 | 1280 | 2560 | 320 | 1280 | 320 | E1/E2 |
| H7N9 | A/Shanghai/7/2013 | 320 | 160 | 320 | 640 | 1280 | 320 | 1280 | 640 | EX/E1 |
| H7N9 | A/Hong Kong/2212982/2014 | 640 | 320 | 320 | 1280 | 2560 | 320 | 1280 | 640 | M1/E1 |
| H7N9 | A/Hong Kong/734/2014 | 320 | 160 | 320 | 1280 | 2560 | 320 | 1280 | 640 | M1/E1 |
| H7N9 | A/Hong Kong/56/2015 | 320 | 80 | 320 | 320 | 1280 | 160 | 640 | 320 | OR/E1 |
| H7N9 | A/Hong Kong/2550/2015 | 160 | 80 | 160 | 320 | 1280 | 160 | 1280 | 320 | C1/E1 |
| H7N9 | A/British Columbia/2015 | 640 | 320 | 320 | 1280 | 1280 | 320 | 1280 | 1280 | C1/C1 |
a E—eggs, SPF–special pathogen free eggs, C–cells, M–MDCK cells, OR–original specimen
b Homologous titers of ferret antiserum to reference antigen are indicated in bold
^ The complete names of the antigen are listed under the reference strains.