A flow diagram on the successful implementation of human immunodeficiency virus (HIV) and tuberculosis (TB) novel diagnostics in resource-limited settings and the required consideration of a number of factors within specific context. Factors include the engagement of all stakeholders, the dissemination of knowledge on results interpretation, the improvement of healthcare systems’ management practices, the support from and to laboratories and clinics, the integration of HIV and TB diagnostics, and the enhancement of linkage to healthcare.