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Multiple reaction monitoring (MRM) conditions for the LC-ESI-MS/MS analysis of OAs.
| OA | Retention time (min) | MRM transitions; precursor > product ions | Cone voltage (V) | Collision energy (V) |
|---|---|---|---|---|
| Tartaric acid | 1.15 | 149 > 87 + 73 | 35 | 12 |
| Malic acid | 1.30 | 133 > 115 + 71 | 35 | 12 |
| Malonic acid | 1.45 | 103 > 59 | 35 | 6 |
| Citric acid | 1.91 | 191 > 111 + 87 | 35 | 18 |
| Succinic acid | 2.43 | 117 > 73 + 99 | 35 | 12 |
| IS succinic acid-d4 | 2.39 | 121 > 77 + 102 | 35 | 12 |
