Table 2.
Investigation of mutant protein stability by I-Mutant 2.0.
| Protein symbol | Amino acid change | Amino acid position | Reliability index (RI) | DDG value (kcal/mol) | Stability prediction |
|---|---|---|---|---|---|
| Slc11a2 | V/I | 108 | 7 | 0.86 | Decrease |
| I/T | 114 | 3 | − 2.03 | Decrease | |
| V/A | 334 | 9 | − 2.13 | Decrease | |
| T/K | 336 | 4 | − 0.48 | Decrease | |
| T/A | 343 | 8 | − 2.05 | Decrease | |
| G/R | 369 | 2 | − 1.01 | Decrease | |
| A/S | 371 | 7 | − 0.81 | Decrease | |
| Y/C | 374 | 1 | 0.71 | Decrease | |
| A/V | 377 | 2 | 0.06 | Decrease | |
| Q/H | 385 | 3 | 0.27 | Decrease | |
| M/V | 389 | 6 | − 0.17 | Decrease | |
| R/Q | 465 | 8 | − 1.88 | Decrease | |
| W/L | 477 | 6 | 1.00 | Decrease | |
| L/V | 484 | 9 | − 1.07 | Decrease | |
| S/F | 490 | 0 | − 0.88 | Decrease | |
| N/S | 492 | 6 | − 0.88 | Decrease | |
| V/M | 497 | 8 | − 1.28 | Decrease | |
| D/G | 502 | 7 | − 2.14 | Decrease | |
| V/A | 507 | 9 | − 2.93 | Decrease | |
| V/M | 510 | 8 | − 2.69 | Decrease | |
| A/V | 512 | 1 | 0.76 | Decrease | |
| V/I | 517 | 9 | − 1.61 | Decrease |