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. 2015 Jul 10;5:309–313. doi: 10.1016/j.gdata.2015.06.026
Specifications
Organism/cell line/tissue Human (adult and fetal ovary), marmoset (adult testis and ovary), cattle (adult ovary, oocytes, cumulus and in vitro fertilized 2–4 cell stage embryos)
Sex Male and female
Sequencer or array type Illumina HiSeq 2500
Data format Raw and analyzed
Experimental factors Normal and untreated tissues/cells
Experimental features Sequencing of small RNA transcriptomes. Comparison of small RNA libraries with and without a NaIO4 oxidation step (for selected samples).
Consent Human adult ovary samples were from cancer patients that underwent unilateral oophorectomy for fertility preservation and have signed informed consent. The human fetal material used was from elective abortions and donated for research with informed consent. The research on human material was approved by the Medical Ethical Committee of the Leiden University Medical Center (CME P08.087 and CME 05/03 K/YR).
Sample source location Leiden, Netherlands (human samples), Göttingen, Germany (macaque samples), Utrecht, Netherlands (bovine samples)