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Technical DNA methylation validation.
(A) Scatter plot of DNA methylation levels obtained by Infinium Human Methylation 450k BeadChip (Illumina Inc., San Diego, CA) (Y-axis) and bisulfite pyrosequencing (X-axis) (n = 11). A highly significant correlation (P < 0.001, R2 = 0.978 and R2 = 0.968, respectably) between microarray and pyrosequencing data was observed. Example of pyrograms with methylated (B) and unmethylated (C) cytosines.
