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. Author manuscript; available in PMC: 2016 Sep 24.
Published in final edited form as: Cell. 2015 Sep 24;163(1):55–67. doi: 10.1016/j.cell.2015.09.004

Figure 6. Molecular Signature of oRG Cells Emerges in VZ.

Figure 6

(A) In situ hybridization for genes enriched in oRG cells across multiple developmental time points corresponding to human cortical neurogenesis. Sections were stained with antibodies against SOX2, EOMES and SATB2 to identify major cell populations (see also Figure S4 and S7). (B) The proportion of oRG marker cells that express all combinations of protein markers was quantified across 10 bins that span the germinal zone throughout neurogenesis. In all cases, oRG marker transcripts are almost exclusively expressed by SOX2+/EOMES–/SATB2– cells (black bars). Bar graphs highlight that at GW13.5 oRG markers predominantly label radial glia in the VZ, but by GW14.5 oRG markers predominantly label radial glia cells in the SVZ. (C) The proportion of SOX2+/EOMES–/SATB2– that expressed TNC was quantified across the span of the germinal zone at GW13.5 and GW18. (D) Immunolabelling of early developmental time points for oRG markers and SOX2. At GW13.5, TNC and PTPRZ1 protein show limited expression in radial glia close to the ventricular edge, while FAM107A protein is widely expressed in GW13.5 VZ. See also Figure S7.