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Primers and probes designed for detection of CGRP and substance P
| CGRP | |
|---|---|
| Accession number | AF330212 |
| Forward primer (position 302) | 5’-AGGAGGCTGAGGGCTCTAGTG-3’ |
| Reverse primer (position 358) | 5’- CAGCCGATGGGTCACACA-3’ |
| Internal probe (FAM-labeled) | 5’-CTGCTCAGAAGAGATCCTGCAACACTGC-3’ |
| Substance P | |
| Accession number | NM_009311 |
| Forward primer (position 212) | 5’-GACCAGATCAAGGAGGCAATG-3’ |
| Reverse primer (position 260) | 5’-GGGTCTTCGGGCGATTCT-3’ |
| Internal probe (TET-labeled) | 5’-CGGAGCCCTTTGAGCATCTTCTGCA-3’ |
