Fig. 5.

Impact of KIEs on the distribution of isotopes in intracellular metabolites. The impact of KIEs on the different isotopic datasets of central metabolites (isotopologue abundances in panels a-c, isotopomer abundances in panels d-f and enrichments in panels g-i) was simulated for three label inputs: glucose at natural abundance, 80 % 1-¹³C-glucose + 20 % U-¹³C-glucose, and 50 % ¹²C-glucose + 50 % U-¹³C-glucose. Error is defined as the absolute difference between isotopic data simulated with and without KIEs, i.e. with alpha coefficients set to their experimental values (Table 1) or to 1, respectively