Table 1a.
Primers used for the amplification of BRAF, NRAS, C-KIT, PPP6C, RAC1 and STK19 genes.
| Gene | Primer | Exon | Primer Sequence | Length of product (bp) |
|---|---|---|---|---|
| BRAF | Forward | 11 | 5′ TTTCTTTTTCTGTTTGGCTTG | 200 |
| BRAF | Reverse | 11 | 5′ ACTTGTCACAATGTCACCACA | 200 |
| BRAF | Forward | 15 | 5′ TGCTTGCTCTGATAGGAAAA | 204 |
| BRAF | Reverse | 15 | 5′ TCAGTGGAAAAATAGCCTCA | 204 |
| NRAS | Forward | 2 | 5′ CGCCAATTAACCCTGATTAC | 201 |
| NRAS | Reverse | 2 | 5′ AGAGACAGGATCAGGTCAGC | 201 |
| NRAS | Forward | 3 | 5′ CCCCTTACCCTCCACACC | 245 |
| NRAS | Reverse | 3 | 5′ AACACAAAGATCATCCTTTCAGA | 245 |
| C-KIT | Forward | 11 | 5′ TGTTCTCTCTCCAGAGTGCTCTAA | 291 |
| C-KIT | Reverse | 11 | 5′ AAACAAAGGAAGCCACTGGA | 291 |
| PPP6C | Forward | 7 | 5′ AAACTCATCTGCAGAGCACA | 271 |
| PPP6C | Reverse | 7 | 5′ AAGAAGAGGGCAGAAAAATG | 271 |
| RAC1 | Forward | 2 | 5′ TGTGATGTATATGCCTTGATTTT | 254 |
| RAC1 | Reverse | 2 | 5′ AGCAAAACAAATGGTCAAAG | 254 |
| STK19 | Forward | 2 | 5′ GACAAGTTGACGCTCCTTTC | 270 |
| STK19 | Reverse | 2 | 5′ AGAGGATCCGACTCCACAG | 270 |