Figure 4.

CADM1 is a novel target of miRNA-126. A. Predicted consequential pairing of target 3’-UTR region of CADM1 (wild type or mutated) and miR-126 mature sequence. B. GES-1 cells were transiently cotransfected with luciferase report vectors, and either miR-126 mimics or miR-control. Luciferase activities were normalized to the activity of Renilla luciferase. C and D. GES-1 cells were transfected with the GFP report vectors and either miR-126 mimics or miR-control. E. Western blots analysis for CADM1 of SGC-7901 cells transfected with indicated oligonucleotides. Data are representative of at least three independent experiments, *P < 0.05.