Figure 4.

A. The effect of miR-34a on the apoptosis of gastric cancer cells after transfection with miR-34a mimic. Cells were stained with PI and analyzed by flow cytometry at 48 h post-transfection. Cells staining positive for Annexin-V-FITC and negative for PI at 48 h post-transfection were considered to have undergone apoptosis. B, C. The relative expression of Tgif2 in gastric cancer transfected with Tgif2 siRNA and the controls after transfection with Tgif2 siRNA. B: real-time PCR; C: western blot analysis.