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Nested PCR primer sequence
| Target gene | Primer | Sequence (5’->3’) |
|---|---|---|
| CD11a (1st round) | Forward | GGTGAATTCTTAAGGTTAGGAGTTTAAGTTTAGT |
| Reverse | CAATCTAGAACTACAACTTAAAAAATTAAATTGC | |
| CD11a (2nd round) | Forward | GTTGAATTCGGTATATATGGTGAAATTTACTTTTAT |
| Reverse | CACTCIAGTACAACAAACATCCAAAAATATAAAAT | |
| CD70 (1st round) | Forward | GGTGAATTCTTAAAGGTTAGGAGTTCAAGTTTAGT |
| Reverse | CAACCTAGAACTACACATTCATTAAAAATTAAATT | |
| CD70 (2nd round) | Forward | GTTGAACTCGGTTAACTGGTGAAATTTTATTTTCAT |
| Reverse | CACTCTAGATACAACAAACATCCAAAAATTAAAAAT |
