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Real-time PCR was performed to detect the mRNA level of HIF-1α and survivin in the all experiment groups. Expression of HIF-1α was increased in the DM3 and DM6 groups compared to that in normal group, and survivin expression was increased significantly in the DM6 groups. β-actin served as internal control. *P<0.05, **P<0.01 visa to normal group.
