Skip to main content
. 2015 Aug 1;8(8):9376–9382.

Figure 2.

Figure 2

The effect of miR-184 on the expression of tumor related gene and the activity of signal pathway of human glioma and breast cancer cells. A. Real-time PCR assay results showed that there was an increased mRNA expression expression of p53 and p21 and there was a decreased mRNA expression of SND1, MMP-2/9 and CD44 in human glioma U87MG and breast cancer MCF-7 cell line after miR-184 expression. B. Western blotting assay results showed that there was an increased protein expression expression of p53 and p21 and there was a decreased protein expression of SND1, MMP-2/9 and CD44 in human glioma U87MG and breast cancer MCF-7 cell line after miR-184 expression. C. ELIASA assay results showed that there was an increased activity of caspase-3/8 in human glioma U87MG and breast cancer MCF-7 cell line after miR-184 expression. D. Western blotting assay showed that there was a decreased the phosphorylation of AKT and report gene assay showed that there was a decreased activity of NF-κB in human glioma U87MG and breast cancer MCF-7 cell line after miR-184 expression.