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. 2015 Sep 25;11(9):e1005192. doi: 10.1371/journal.ppat.1005192

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© 2015 Park et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) The concentrations of metal ions in CabA analyzed by ICP-AES. ND, non-detection. (B) Typical isothermal titration calorimetric measurement of the calcium binding to CabA. The raw data displayed in the upper panel were integrated in the lower panel. The data points represent the experimental injection heat and the solid line corresponds to the calculated fit of the data using a model with two sets of binding sites of the ORIGIN software package (MicroCal Inc.). The heat data of calcium into the reaction buffer was subtracted from the reaction heat data between CabA and calcium. The experimental parameters were as follows: 70 injections of 3 μl of calcium solution, stirring speed of 307 rpm, duration 12 s, and spacing 240 s. (C) Thermodynamic parameters of the calcium binding to CabA.