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. 2015 Sep 25;11(9):e1005192. doi: 10.1371/journal.ppat.1005192

Fig 3. Intracellular levels of c-di-GMP and cabA expression.

Fig 3

The strains were grown to A 600 of 0.15 with VFMG-CF containing 10 mM of CaCl2, and different concentrations of arabinose as indicated. (A) Intracellular levels of c-di-GMP were measured using LC-MS. (B) Transcripts of cabA were determined using qRT-PCR. Relative levels of the c-di-GMP and cabA mRNA were presented as those levels in JN111 grown without arabinose as 1, respectively. *, P<0.05; **, P<0.005 relative to the c-di-GMP or cabA mRNA levels in JN111 grown without arabinose. Error bars represent the SD. (C) Protein samples were resolved on SDS-PAGE and immunoblotted using the rabbit anti-CabA antibody. The protein size markers (Precision Plus Protein Standards; Bio-Rad Laboratories) and CabA (arrows) are shown in kDa. CMCP6, wild type; JN111, parent strain; cabA, cabA mutant.