Fig 7. Expression of CabA at the different development stages of biofilm.

(A) Biofilm development of JN111 and the cabA mutant with VFMG-CF containing 10 mM of CaCl₂ and 0.01% of arabinose was monitored using CV staining. Total RNAs and proteins were prepared from the JN111 biofilms (that is, from the cells and matrices) harvested at different stages as indicated by vertical arrows. *, P<0.05; **, P<0.005 relative to the JN111 biofilm. JN111, parent strain; cabA, cabA mutant. (B) The cabA mRNA levels were determined by qRT-PCR analyses, and the cabA mRNA level in the biofilms harvested after 2 h incubation was set as 1. **, P<0.005 relative to the cabA mRNA level in the biofilms harvested after 2 h incubation. Error bars represent the SD. (C) Protein samples were resolved on SDS-PAGE and immunoblotted using the rabbit anti-CabA antibody. The protein size markers (Precision Plus Protein Standards; Bio-Rad Laboratories) and CabA (horizontal arrow) are shown in kDa.