Fig 8. Effects of the cabA mutation on colony morphology.

(A) The strains were spotted onto VFMG-CF agar plates supplemented with or without 0.02% of arabinose and 10 mM of CaCl₂. Then, the plates were incubated at 30°C for 3 d. Ampicillin and kanamycin (200 μg/ml for each) were used to maintain plasmids in the strains. Each colony representing the mean rugosity of colony morphology from at least three independent experiments was visualized using a stereomicroscope (Stemi DV4, Zeiss) at an 8× magnification. Bars, 1 mm; JN111 (pJK1113), parent strain; cabA (pJK1113), cabA mutant; cabA (pYM1109), complemented strain.