Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics a | Reference or source |
|---|---|---|
| Bacterial strains | ||
| V. vulnificus | ||
| CMCP6 | Wild type V. vulnificus, virulent | Laboratory collection |
| YM112 | CMCP6 with ΔcabA | This study |
| JN111 | CMCP6 with PBAD-dcpA | This study |
| YM112D | JN111 with ΔcabA | This study |
| JN151D | JN111 with ΔcabB | This study |
| JN141D | JN111 with ΔcabC | This study |
| JN094D | JN111 with ΔbrpA::nptI; Kmr | This study |
| JN143D | JN111 with ΔcabA, ΔbrpA::nptI; Kmr | This study |
| E. coli | ||
| BL21 (DE3) | F- ompT hsdS B (rB -, mB -) gal dcm (DE3) | Laboratory collection |
| DH5α | supE44 ΔlacU169 (ϕ80lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1; plasmid replication | Laboratory collection |
| S17-1λpir | λ-pir lysogen; thi pro hsdR hsdM + recA RP4-2 Tc::Mu-Km::Tn7;Tpr Smr; host for π-requiring plasmids; conjugal donor | [63] |
| Plasmids | ||
| pDrive | PCR product cloning vector; Apr Kmr | Qiagen |
| pDM4 | Suicide vector; ori R6K; Cmr | [56] |
| pBAD24 | Expression vector with the PBAD promoter; Apr | [49] |
| pJN1105 | pDrive with PdcpA-dcpA; Apr Kmr | This study |
| pJN1106 | pDrive with PdcpA-PBAD-dcpA; Apr Kmr | This study |
| pJN1107 | pDM4 with PdcpA-PBAD-dcpA; Cmr | This study |
| pET28a(+) | His-tag protein expression vector; Kmr | Novagen |
| pYM1202 | pET28a(+) with cabA; Kmr | This study |
| pYM1102 | pDM4 with ΔcabA; Cmr | This study |
| pJN1504 | pDM4 with ΔcabB; Cmr | This study |
| pJN1402 | pDM4 with ΔcabC; Cmr | This study |
| pJN0907 | pDM4 with ΔbrpA::nptI; Cmr, Kmr | This study |
| pJK1113 | pKS1101 with nptI; Apr Kmr | [57] |
| pYM1109 | pJK1113 with cabA; Apr Kmr | This study |
| pJN1502 | pJK1113 with cabB; Apr Kmr | This study |
| pJN1403 | pJK1113 with cabC; Apr Kmr | This study |
a Kmr, kanamycin resistant; Tpr, trimethoprim resistant; Smr, streptomycin resistant; Cmr, chloramphenicol resistant; Apr, ampicillin resistant.