Table 6.
List of several glycoside hydrolases (GHs) identified in various Anoxybacillus genomes
| GH | Enzyme | Similarity within Anoxybacillus genomea | Number of studied enzymeb | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AB04T | WK1 | E13T | SK3-4 | DT3-1 | TNO | G10 | G2T | AF/04T | AK1 | BCO1 | KU2-6 | Kn10 | PS2 | |||
| 1 | β-glucosidase | 91.2 | 100 | 90.7 | 39.5 | 92.4 | - | - | - | - | - | - | 91.6 | 92.0 | - | - |
| 10 | Endo-1,4-β-xylanase | 100.00 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 13 | α-amylase (cell-bound) | 98.6 | 100.00 | 98.4 | 97.4 | 96.2 | 97.2 | 96.2 | 98.2 | 98.6 | 94.5 | 76.6 | 98.8 | 99.8 | 84.3 | 2 [16] |
| 13 | α-amylase (extracellular) | 77.6 | - | 100.00 | - | - | 95.0 | - | - | - | 54.2 | - | - | - | - | |
| 13 | Pullulanase | 93.4 | 100.00 | 93.9 | 90.5 | 89.5 | 95.6 | 89.4 | 91.9 | 93.2 | 88.9 | 59.9 | 94.8 | 98.0 | 67.4 | 1 [17] |
| 13 | Amylopullulanase (>200 kDa) | - | 100.00 | 90.2 | 88.8 | - | - | 99.1 | - | - | 59.6 | - | - | 89.6 | - | 1 [18] |
| 13 | Amylopullulanase (<200 kDa) | - | - | - | - | - | - | - | - | - | - | 100 | - | - | - | - |
| 13 | CDase | 95.8 | 100.00 | 95.6 | 92.5 | 92.0 | 94.7 | 92.3 | 95.1 | 94.9 | 93.6 | 96.6 | 95.9 | 98.1 | 78.8 | 1 [19] |
| 13 | Oligo-1,6-glucosidase | 98.2 | 100.00 | 61.7 | 96.0 | 96.0 | 98.1 | 96.0 | 53.9 | 97.5 | 97.0 | 53.6 | 96.5 | 98.6 | 90.9 | - |
| 13 | Trehalose-6-phosphate hydrolase | 95.6 | 100.00 | - | 94.2 | 93.7 | - | 93.9 | - | 96.2 | 94.9 | - | 95.8 | 99.1 | - | - |
| 13 | 1,4-α-glucan branching enzyme | 93.4 | 100.00 | - | 93.2 | 92.8 | 94.1 | 92.4 | - | 93.9 | 94.9 | - | - | - | - | |
| 31 | α-glucosidase | 92.1 | 100.00 | 92.9 | 91.0 | 89.2 | 88.1 | 89.2 | 91.5 | 91.7 | 88.5 | 71.1 | 93.4 | 96.9 | 67.4 | - |
| 32 | Sucrase-6-phosphate hydrolase | 94.7 | 100.00 | - | 91.3 | 91.8 | 91.0 | 91.3 | - | 93.5 | 92.5 | - | 93.9 | 93.3 | - | - |
| 36 | α-galactosidase | - | - | 91.2 | - | - | - | 79.2 | 100 | - | 90.5 | 72.3 | 93.7 | - | 79.5 | - |
| 51 | α-L-arabinofuranosidase | 93.6 | 100.00 | - | - | - | - | - | - | - | 99.4 | - | - | - | - | 1 [22] |
| 52 | β-xylosidase | 91.5 | - | 90.4 | - | - | - | 100 | 99.6 | - | - | - | - | 89.5 | - | |
| 65 | Sugar hydrolase/phosphorylase | - | 100.00 | - | 94.9 | 94.1 | - | 94.0 | - | 94.1 | - | - | 96.6 | - | - | |
| 67 | α-glucuronidase | 100.00 | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
aThe reference for the protein sequence alignment is denoted as 100 %; bThe numbers represent the respective cloned, purified, and characterized enzymes from Anoxybacillus species. AB04T = A. ayderensis AB04T [30]; WK1 = A. flavithermus WK1 [5, 29]; E13T = A. flavithermus subsp. yunnanensis E13T [35, 47, 48]; SK3-4 = Anoxybacillus sp. SK3-4 [45, 46]; DT3-1 = Anoxybacillus sp. DT3-1 [45, 46]; TNO = A. flavithermus TNO-09.006 [5, 44]; G10 = A. kamchatkensis G10 [40–42]; G2T = A. gonensis G2T [36]; ATT = A. thermarum AF/04T [33–35]; AK1 = A. flavithermus AK1 [5, 39]; BCO1 = Anoxybacillus sp. BCO1 [31, 32]; KU2-6 = Anoxybacillus sp. KU2-6(11); Kn10 = A. flavithermus Kn10 [5, 43]; PS2 = A. tepidamans PS2 [37]