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. 2015 Sep 13;2015:712438. doi: 10.1155/2015/712438

Figure 2.

Figure 2

TMEM132D is overexpressed in TIL+ early stage ovarian cancer and its expression correlates positively with CD8+ T-lymphocyte infiltration. (a) TMEM132D was overexpressed in the TIL+ ovarian cancer sample as visualized by fluorescent ADDER (band indicated by an arrow). (b) The expression of GPC6 was quantified by qPCR in samples derived from 38 stage I-II ovarian cancer patients. The relative expression of TMEM132D was plotted along with the respective CD8A expression levels after normalization to b-actin. A pooled mix of equal amounts of all samples served as calibrator (Cal). (c) Pearson correlation coefficient of CD8A-TMEM132D expression levels. The correlation is significant (p = 0.0067). (d) The relative expression of TMEM132D in 7 ovarian cancer cell lines was estimated by qPCR (normalized to b-actin). (e-f) Immunohistochemistry of TMEM132D expression in representative early stage ovarian tumor samples. (e) Negative expression of TMEM132D protein in the tumor sample 408 with low mRNA levels of the respective gene (see 2(b)). (f) Very strong expression of the TMEM132D protein in the 314 sample with also high mRNA levels (see 2(b)). Magnification (e-f): ×400. Columns: mean, bars: SEM.