Table 1. PCR Primers and the Annealing Temperature Used in This Study.
| Gene | Primer | Amplicon Size | Annealing |
|---|---|---|---|
| bla OXA-23F | GAT CGG ATT GGA GAA CCAGA | 501 bp | 53 |
| bla OXA-23R | ATT TCT GAC CGC ATT TCC AT | ||
| bla OXA-24F | GGT TAG TTG GCC CCC TTA AA | 246 bp | 53 |
| bla OXA-24R | AGT TGA GCG AAA AGG GGA TT | ||
| bla OXA-58F | AAG TAT TGG GGC TTG TGC TG | 599 bp | 53 |
| bla OXA-58R | CCCCTCTGCGCTCTACATAC | ||
| Isaba-1(F) | CACGAATGCAGAAGTTG | 1200 bp | 49 |
| OXA-23-R | TTAAATAATATTCAGCTGT | ||
| Int-1F | CAGTGGACATAAGCCTGTTC | 160 bp | 62 |
| Int-1R | CCCGAGGCATAGACTGTA | ||
| Int-2F | GTAGCAAACGAGTGACGAAATG | 788 bp | 62 |
| Int-2R | CACGGATATGCGACAAAAAGGT | ||
| Int-3F | GCCTCCGGCAGCGACTTTCAG | 979 bp | 62 |
| Int-3R | ACGGATCTGCCAAACCTGACT |