Table 2.
Average proportions of cell types on day 1 with comparisons to control values.
| Radial glial progenitors |
Neurons |
Unclassified |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Morpholino (N) | %± SEM | Mann-Whitney unpaired p- values |
% Of control levels ± SEM |
%± SEM | Mann-Whitney unpaired p- values |
% Of control levels ± SEM |
%± SEM | Mann-Whitney unpaired p- values |
% Of control levels ± SEM |
| conMO Group 1 (11) | 43.1 ± 3.4 | - | 99.9 ± 8 | 39.8 ± 3.4 | - | 100 ± 8.6 | 17 ± 4.1 | - | 100 ± 24 |
| arl9 (8) | 51.7 ± 3.4 | 0.5889 | 93.5 ± 6.2 | 41 ± 4.4 | 0.3347 | 114 ± 12.4 | 7.1 ± 3.4 | 0.7803 | 82.5 ± 39.9 |
| armc8 (8) | 54.8 ± 6.6 | 0.6158 | 99.1 ± 11.9 | 29.8 ± 9 | 0.2468 | 82.8 ± 25 | 15.3 ± 4.5 | 0.2407 | 177 ± 52.9 |
| chn1 (7) | 59.3 ± 5.6 | 0.5538 | 107.2 ± 10.1 | 19.9 ± 2.2 | 0.0052 | 55.3 ± 6.1 | 20.7 ± 4.6 | 0.0218 | 239.5 ± 53.9 |
| ctdnep1-a (8) | 70.1 ± 5.5 | 0.0038 | 126.6 ± 9.9 | 22.5 ± 6.5 | 0.0474 | 62.7 ± 18.1 | 7.3 ± 2.3 | 0.1157 | 84.5 ± 27.6 |
| eif3a (8) | 65.8 ± 8.4 | 0.1322 | 119 ± 15.2 | 20.5 ± 6.1 | 0.0252 | 57.1 ± 17.1 | 13.5 ± 3.4 | 0.2763 | 156.2 ± 40.2 |
| fbxw7 (8) | 54.7 ± 2.2 | 0.9385 | 98.8 ± 3.9 | 30.6 ± 2.6 | 0.5627 | 84.9 ± 7.2 | 14.7 ± 2.9 | 0.1519 | 169.9 ± 34 |
| fgf2 (9) | 50.4 ± 4.4 | 0.4136 | 91.2 ± 7.9 | 37.1 ± 4.4 | 1 | 103.1 ± 12.3 | 12.3 ± 2.5 | 0.2106 | 143.1 ± 29.3 |
| glis2 (8) | 59.5 ± 6.8 | 0.6159 | 107.6 ± 12.3 | 30.4 ± 3.9 | 0.5883 | 84.5 ± 10.9 | 10 ± 3.4 | 0.7544 | 115.7 ± 40 |
| hat1 (8) | 52.7 ± 3.7 | 0.7574 | 95.2 ± 6.6 | 27.8 ± 4.6 | 0.4177 | 77.3 ± 12.9 | 19.4 ± 2.8 | 0.0247 | 224.7 ± 32.5 |
| lsm6 (8) | 58.5 ± 3.6 | 0.9692 | 105.8 ± 6.6 | 25.2 ± 2.9 | 0.0825 | 70.1 ± 8.2 | 16.1 ± 1.7 | 0.0223 | 186.8 ± 20.2 |
| mecp2 (8) | 38.8 ± 4.5 | 0.015 | 70.1 ± 8.1 | 48.3 ± 3.8 | 0.0371 | 134.3 ± 10.5 | 12.8 ± 2.2 | 0.1752 | 148.1 ± 25.6 |
| MGC81190/actn1 (9) | 59.7 ± 3.5 | 0.3552 | 107.9 ± 6.5 | 26.9 ± 2.7 | 0.145 | 74.9 ± 7.7 | 13.2 ± 2.4 | 0.1524 | 153.3 ± 28.2 |
| mkrn2 (8) | 50.2 ± 7.3 | 0.3742 | 90.7 ± 13.2 | 40.2 ± 6.1 | 0.4174 | 111.8 ± 16.9 | 9.5 ± 3.5 | 0.9377 | 109.7 ± 40.9 |
| mocs3 (9) | 62 ± 6.6 | 0.5218 | 112.1 ± 12 | 27.1 ± 4.8 | 0.3369 | 75.2 ± 13.4 | 10.8 ± 3.5 | 0.6121 | 125.3 ± 41.1 |
| pura (8) | 57 ± 7.4 | 1 | 103.1 ± 13.4 | 36.5 ± 6.3 | 0.7871 | 101.5 ± 17.7 | 6.3 ± 2.7 | 0.55 | 73.5 ± 31.5 |
| r3hdm2 (8) | 62 ± 5.1 | 0.2027 | 112 ± 9.2 | 28.1 ± 4.8 | 0.2797 | 78.2 ± 13.4 | 9.8 ± 1.9 | 0.5599 | 113.4 ± 22.4 |
| rbfox2b (9) | 42.6 ± 5.2 | 0.0593 | 76.9 ± 9.5 | 47.7 ± 5.7 | 0.081 | 132.6 ± 15.9 | 9.6 ± 1.8 | 0.5914 | 111.3 ± 21.3 |
| slc12a2 (9) | 54.8 ± 6.7 | 0.6958 | 99 ± 12.2 | 27.8 ± 4.6 | 0.3026 | 77.5 ± 13 | 17.2 ± 4.2 | 0.0998 | 199.5 ± 48.5 |
| tle1 (8) | 46 ± 3.6 | 0.0825 | 83.1 ± 6.5 | 43.9 ± 3.1 | 0.1135 | 121.9 ± 8.8 | 10 ± 0.9 | 0.4385 | 116.1 ± 10.6 |
| vangl1 (7) | 51.7 ± 3.9 | 0.4465 | 93.5 ± 7.1 | 32 ± 4.3 | 0.7036 | 89 ± 12.1 | 16.1 ± 2.5 | 0.0745 | 186.6 ± 29.2 |
| conMO Group 2 (12) | 55.3 ± 3.3 | - | 100 ± 6.1 | 35.9 ± 3.7 | - | 100 ± 10.4 | 8.6 ± 2.6 | - | 99.9 ± 31.1 |
| cpeb1-a (10) | 32.2 ± 4.4 | 0.0317 | 74.7 ± 10.3 | 54.7 ± 3.5 | 0.0112 | 137.5 ± 8.9 | 13 ± 2.2 | 1 | 76.3 ± 13.4 |
| dio3 (9) | 42.6 ± 8.2 | 0.9092 | 98.8 ± 19.1 | 42.5 ± 6.7 | 0.8197 | 106.9 ± 16.8 | 14.7 ± 2.7 | 0.7034 | 86.7 ± 16.3 |
| efna3 (6) | 29.7 ± 7.7 | 0.1445 | 68.8 ± 18 | 57.2 ± 7.4 | 0.0786 | 143.7 ± 18.6 | 13 ± 4.6 | 0.8798 | 76.7 ± 27.4 |
| elk4 (11) | 27.9 ± 4.8 | 0.0126 | 64.8 ± 11.3 | 47.4 ± 4.8 | 0.2638 | 119.1 ± 12 | 24.5 ± 3.9 | 0.0817 | 144.4 ± 23.1 |
| ephb1 (5) | 56.3 ± 11.5 | 0.3349 | 130.4 ± 26.8 | 16 ± 6.3 | 0.0127 | 40.2 ± 16 | 27.6 ± 8.9 | 0.2811 | 162.3 ± 52.6 |
| epx (14) | 36.8 ± 3.5 | 0.1887 | 85.3 ± 8.2 | 40.4 ± 4.5 | 0.8911 | 101.5 ± 11.4 | 22.7 ± 3.4 | 0.171 | 133.4 ± 20.5 |
| fmr1-a (8) | 50.5 ± 11.5 | 0.9341 | 117 ± 26.6 | 25.8 ± 7.1 | 0.1264 | 65 ± 18 | 23.5 ± 7.4 | 0.6494 | 138.4 ± 43.8 |
| fxr1-a (10) | 36 ± 7.9 | 0.0778 | 83.5 ± 18.3 | 39.8 ± 8.2 | 0.9438 | 100.1 ± 20.6 | 24 ± 7.6 | 0.7245 | 141.4 ± 44.7 |
| gstp1 (7) | 12.4 ± 4.2 | 0.0011 | 28.8 ± 9.8 | 74.1 ± 4.4 | 0.0006 | 186.3 ± 11 | 13.3 ± 3.6 | 0.4685 | 78.5 ± 21.6 |
| hdac6 (6) | 15.7 ± 6.1 | 0.0103 | 36.5 ± 14.2 | 61.7 ± 6.8 | 0.0307 | 155.1 ± 17.2 | 22.4 ± 7.4 | 0.2079 | 132 ± 43.8 |
| hspa5 (7) | 46.2 ± 8 | 0.8207 | 107 ± 18.7 | 45.3 ± 6.4 | 0.6505 | 113.8 ± 16.1 | 8.5 ± 2.7 | 0.2364 | 49.8 ± 15.9 |
| mmp9 (6) | 25.3 ± 7.9 | 0.1188 | 58.5 ± 18.4 | 56.6 ± 7.2 | 0.0873 | 142.3 ± 18.2 | 18 ± 4 | 0.546 | 106 ± 23.5 |
| prkaca (6) | 22 ± 8.3 | 0.0267 | 51 ± 19.4 | 61.2 ± 8.7 | 0.0347 | 153.9 ± 21.8 | 16.7 ± 3.2 | 0.6507 | 98.1 ± 18.8 |
| Wnt7b (8) | 46.8 ± 4.6 | 0.5913 | 108.6 ± 10.8 | 37.6 ± 5.6 | 0.4818 | 94.6 ± 14.1 | 15.4 ± 3.2 | 0.7097 | 90.5 ± 19.2 |
| noMO Control (13) | 35.3 ± 4.4 | 0.2022 | 81.8 ± 10.3 | 45.6 ± 3.5 | 0.4512 | 114.7 ± 8.8 | 19 ± 4.3 | 0.9077 | 111.6 ± 25.7 |
| conMO Combined (23) | 49.5 ± 2.7 | - | - | 37.8 ± 2.5 | - | - | 12.67 ± 2.52 | - | - |
Each control morpholino group (conMO group 1 and group 2) is compared to the experimental MO data acquired over the same period (experimental results grouped below each control group). All pSox2-bd::FP labeled cells in a tectal lobe were categorized as neurons, radial glial progenitors or unclassifiable/undifferentiated cells and the mean percent of each cell type and standard error of the mean (SEM) are given. The % of control values ± SEM is calculated by dividing the cell percentage of the experimental group by the control value. Mann-Whitney Unpaired tests were conducted for differences in the percent of each cell type compared to control values and the p-values are given. Significant differences from control values are in bold. N=number of electroporated tecta examined. The “conMO Combined” data averages all control MO values and is displayed in Figs. 7 and 8; it is not used for statistical comparisons.