Table 5.
Average proliferation rates between day 1 and day 3 with comparisons to control values.
| Morpholino | Tectal lobe# | Total cell # | Cell proliferation rate between day 1 and day 3 mean ± SEM | Mann-Whitney Unpaired p-values | % of control cell proliferation rate mean ± SEM |
|---|---|---|---|---|---|
| conMO Group 1 | 12 | 247 | 23.5 ± 5.5 | 0.8052 | 100 ± 23.5 |
| arl9 | 8 | 304 | 73.8 ± 15.4 | 0.005 | 314.8 ± 65.6 |
| armc8 | 8 | 277 | 179.1 ± 49.8 | 0.003 | 763.9 ± 212.7 |
| chn1 | 7 | 371 | 96.4 ± 23.1 | 0.002 | 411.1 ± 98.5 |
| ctdnep1 | 8 | 215 | 97.4 ± 26.9 | 0.006 | 415.5 ± 114.5 |
| eif3a | 8 | 300 | 40.9 ± 8.1 | 0.097 | 174.4 ± 34.5 |
| fbxw7 | 8 | 423 | 50.8 ± 18.4 | 0.202 | 216.4 ± 78.7 |
| fgf2 | 9 | 407 | 64.7 ± 9.4 | 0.002 | 275.9 ± 40.3 |
| glis2 | 8 | 300 | 63.8 ± 15.4 | 0.012 | 271.6 ± 65.7 |
| hat1 | 8 | 417 | 46.9 ± 5.4 | 0.018 | 200 ± 23.1 |
| lsm6 | 8 | 438 | 78.8 ± 16.3 | 0.005 | 336.4 ± 69.9 |
| mecp2 | 8 | 288 | 44 ± 7.3 | 0.058 | 187.8 ± 31.3 |
| MGC8U91/actn1 | 9 | 367 | 69.3 ± 15.1 | 0.006 | 295.7 ± 64.3 |
| mkrn2 | 8 | 221 | 9.3 ± 9.5 | 0.177 | 39.5 ± 40.5 |
| mocs3 | 8 | 238 | 124.5 ± 31.1 | 0.002 | 531.3 ± 132.5 |
| pura | 8 | 232 | 48.1 ± 11.3 | 0.089 | 205 ± 48.2 |
| r3hdm2 | 8 | 278 | 37.9 ± 8.2 | 0.203 | 161.6 ± 34.9 |
| rbfox2b | 9 | 394 | 44.4 ± 21.1 | 0.722 | 189.3 ± 90 |
| slc12a2 | 8 | 309 | 31 ± 6.4 | 0.396 | 132.1 ± 27.1 |
| tle1 | 8 | 300 | 20.1 ± 4.5 | 0.877 | 85.8 ± 19.3 |
| vangl1 | 7 | 237 | 34.4 ± 21.8 | 0.704 | 146.5 ± 92.9 |
| conMO Group 2 | 11 | 296 | 20.8 ± 4.1 | 0.8052 | 100 ± 19.8 |
| cpeb1-a | 10 | 157 | 6.2 ± 3.8 | 0.029 | 29.5 ± 18.7 |
| dio3 | 9 | 104 | 34.8 ± 14.4 | 0.676 | 167.3 ± 69.3 |
| efna3 | 6 | 66 | 12.9 ± 5.1 | 0.159 | 62.1 ± 24.7 |
| elk4 | 11 | 322 | 37.7 ± 8.04 | 0.048 | 181.7 ± 38.7 |
| ephB1 | 5 | 91 | 115.6 ± 30.2 | 0.002 | 556.7 ± 145.5 |
| epx | 14 | 385 | 15.1 ± 4.2 | 0.338 | 73.1 ± 20.4 |
| fmr1-a | 9 | 185 | –10.5 ± 11.2 | 0.033 | –47.8 ± 61.1 |
| fxr1-a | 10 | 101 | –3.2 ± 13.9 | 0.024 | –15.4 ± 66.8 |
| gstp1 | 7 | 163 | 1.5 ± 6.3 | 0.013 | 7.4 ± 30.7 |
| hdac6 | 6 | 172 | 14.4 ± 13.5 | 0.291 | 68.8 ± 65.1 |
| hspa5 | 7 | 121 | –2.6 ± 14.6 | 0.024 | –12.2 ± 70.2 |
| mmp9 | 6 | 117 | –1.8 ± 8.3 | 0.027 | –8.7 ± 39.8 |
| prkaca | 6 | 98 | –10.7 ± 6.4 | 0.003 | –51.5 ± 30.7 |
| wnt7b | 8 | 119 | 11.2 ± 11.6 | 0.231 | 53.8 ± 56.1 |
| noMO Control | 13 | 208 | 52.7 ± 14 | 0.118 | 253.7 ± 67.3 |
| conMO Combined | 23 | 543 | - | 100 ± 15.15 |
Each control morpholino group (conMO group 1 and group 2) is compared to the experimental MO data acquired over the same period (experimental results grouped below each control group). All pSox2-bd::FP labeled cells in a tectal lobe were counted on days 1 and 3 and the mean differences and standard error of the mean (SEM) are given. The % of control levels ± SEM is calculated by dividing the proliferation rate of the experimental group by the mean control proliferation rate. Mann-Whitney Unpaired tests were conducted for differences in the proliferation rates to the control values and the p-values are given. Significant differences from control values are in bold. The “conMO Combined” value is the average averages of all MO control values and is displayed in Figs. 7 and 8, but it is not used for statistical comparisons.