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SDS-PAGE and Western blotting with polyclonal and monoclonal antibodies. (a) the purified polyclonal antibody (lanes 1 and 2) was separated using SDS-PAGE with the molecular mass markers indicated in lane M. (b) PEGX-4T1 control protein (lane 1) and GST-TK fusion protein (lane 2) were transferred onto nitrocellulose membranes for Western blotting using a polyclonal antibody.
