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Inhibitory role of IFNγ on IL-10- and TGFβ-dependent endotoxin tolerance. Monocytes were pretreated or not with 10 ng/mL IFNγ and primed with 10 ng/mL IL-10 (a) or 50 ng/mL TGFβ (b) for 18 h and then stimulated with 10 ng/mL LPS. Supernatants were collected 24 h later; TNFα protein levels were quantified by ELISA. Results are expressed as mean ± SEM of three independent experiments.
