Figure 3. Introducing DSBs into both parental genomes vastly induces homologous recombination in two heteroplasmic lines.
(A) Expression of both mito-BglII and mito-XhoI enzymes in the germline effectively sterilizes most females carrying either mt:ND2del1 or mt:CoIR301Q genome, resistant to only one of the enzymes, but resistant progeny appear at a much higher frequency if the female carries both types of sensitive genomes. The resistant progeny from the heteroplasmic parent are homoplasmic for a newly generated recombinant genotype carrying both parental alleles: mt:ND2del1 + mt:CoIR301Q. (B) The same level of rescue was observed when a different heteroplasmic line was used, which had a different starting ratio of two parental genomes. Results are means ±SD (n = 4 × 100 for each heteroplasmic line).