Fig. 2.

Up-regulation of GAMYB and pollen formation-related genes in the spikelets of HNIL(H143). Relative transcript levels of GAMYB (a), CYP703A3 (Cytochrome P450 hydroxylase) (b), KAR (β-ketoacyl reductase) (c) in HNIL(M23) and HNIL(H143) spikelets were normalized to the transcript levels of UBQ5. The RT-qPCR was performed with total RNA from spikelets at heading stage. The data were obtained from three independent biological replicates. Reactions were repeated at least twice. Student’s t-test was used for statistical analysis (***P < 0.001). Means and standard deviations are recorded as values and vertical bars, respectively