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. 2015 Aug 10;5(3):352–368. doi: 10.3390/membranes5030352

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Selection of functional AqpZ mutants. (A) Screening of a 96-well plate with yeast expressing AqpZ mutants, showing two wells with water-permeable AqpZ (arrows). Conversion of added reagents to orange colour indicates presence of viable cells; (B) Re-screening comparing yeast without AQP or expressing wild type AqpZ (WT), an inactive mutant (R189S) and several inactive/active mutants found; (C-F) Nine functional mutations (yellow), out of 160 mutants carrying 1–5 mutations each, shown on the structure of the AqpZ tetramer: view from (C) cytoplasmic and (D) extracellular side; (E,F) side view (E) and after removing two monomers (F). Results adapted from [60].