Figure 1.

Illustration of the PSLO patterning process for stacked SLBs. Parylene film is deposited onto a clean substrate, and photoresist is patterned using photolithography. After reactive ion etching of Parylene and removal of photoresist, small unilamellar lipid vesicles are deposited on the stencils and incubated to form SLBs. The final step is to lift off the stencil, revealing stacked SLB patterns. Inset: side view illustration of a double lipid bilayer with cationic and anionic lipids, and fluorescence images of a two-bilayer stack.